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仪器名称：半干转膜仪
英文名称：Semi-Dry Blotters
国产/进口：进口
产地/品牌：美国Hoefer
：TE70XP/77XP&
参考报价：询价
总点击数：7192
本半年点击数：699
产品类别：
做WESTERN BLOTTING必不可少的工具！
最大特点：
节约大量的BUFFER,转膜时间比传统湿转快很多。
自带电源和可设定参数，防止过热，无需人工等候。
技术参数：
TE70XP最大转膜面积14X16厘米，77XP最大转膜面积21X26厘米。
浩翰仪器有限公司成立于2003年，由多位生命科学领域专家、业内专业人士组建而成，主要经营生物、医学、制药等实验室科研级仪器，面向祖国大陆和台湾地区，同时出口到欧洲、中东、非洲和西亚等地。
时至今日，我公司以香港为中心，已成立了台北、广州、上海、北京、西安分公司，并在深圳、福州、武汉、长沙、南京等地设有办事处。分实验室基础设备部门和生物技术部门，拥有严密的经营管理，优秀的技术支持，完善的售后服务体系，敏锐的市场洞察力和符合实际的经营策略。
浩翰人追求&精益求精、用心服务&,奉行以客户为中心的理念,以为客户提供优质产品，无忧服务为宗旨，协助客户为生命科学、医学事业的发展尽一份力。
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伯乐Western Blot半干法转膜的十大注意事项
齐一生物科技（上海)有限公司技术部经验分享：首先讲转膜仪的清洗：Do not immerse the unit in liquid. Use special care when cleaning the anode plate to avoid scratching or marring the platinum. Do not use abrasives or strong detergents. The cathode plate (stainless steel) can be cleaned with a mild abrasive to remove salt that may deposit during normal operation. The entire unit can also be periodically disassembled and cleaned with water to remove salt deposits.推荐清洗方法：转膜结束后，立即用双蒸水将转膜仪冲洗几次，并晾干。Electrophoretic transfer of proteins and nucleic acids is dependent on many factors. Observe the following guidelines to avoid mishaps that may result in serious damage to the instrument or injury to the operator.1. Do not reverse polarity on this instrument. This will result in corrosion and rusting of the stainless steel cathode. If this should occur, the stainless steel should be cleaned with a mild abrasive cleaner to remove the rust.2. Do not exeed 25 V with this instrument. This could damage the electrodes.3. Do not adjust the pH of transfer buffers unless specifically indicated. Following instructions carefully. Adjustment of pH of transfer buffers, when not indicated, will result in increased buffer conductivity. This is manifested by a higher than expected initial current output as shown by the power supply's current meter. Monitor buffer resistance with the Model 200/2.0 power supply prior to each run to insure proper buffer conductivity.4. Lengthy tranfer times are not recommended. Do not leave this instrument unattached. Joule heat can be generated rapidly during semi-dry blotting. Transferring longer than 2 hours can damage the unit.5. Power supply requirements. The Trans-Blot SD cell should only be used with the microprocessor-controlled Model 200/2.0 power supply or the Model
power supply.6. Do not operate this instrument in ambient temperatures exceeding 50 ℃.7. Buffer preparation is extremely important. Do not adjust transfer buffer pH by addition of acid or base unless specifically indicated in the instructions. Improperly prepared buffer will cause excess heat generation and safety hazards. Use only high quality, reagent grade methanol. Contaminated methanol can result in increased transfer buffer conductivity, as well as poor transfer of macromolecules.8. Following electrophoresis, equilibrate the gels in transfer buffer. Equilibration facilitates the removal of electrophoresis buffer salts and detergents. If the salts are not removed, they will increase the conductivity of the transfer buffer and the amount of heat generated during the transfer. Also, low percentage gels (&12% acrylamide) will shrink in methanol-containing buffers. Equilibration allows the gel to adjust to its final size prior to electrophoretic transfer. The length of time required for equilibration is dependent on the gel thickness. For example, 15 minutes for a 0.75 mm SDS-PAGE gel.Low molecular weight macromolecules ( 10,000 daltons) may diffuse out of gels more readily. One can allow adequate gel pre-equilibration by changing the pre-equilibration buffer several times during a relatively short pre-equilibration period. This will help to limit diffusion of low molecular weight macromolecules while providing efficient salt reduction.9. Cut the membrane to the dimensions of the gel. Wet the membrane by slowly sliding it at a 45° angle into transfer buffer and allowing it to soak for 15–30 minutes. Complete wetting of the membrane is important to insure proper binding. Abrupt wetting can lead to entrapment of air bubbles in the matrix. These air bubbles can block transfer of molecules. To avoid membrane contamination, always use forceps or wear gloves when handling membranes.10. Cut filter paper to the dimensions of the gel. Two pieces of extra thick filter paper (or four pieces of thick or six pieces of thin filter paper) per gel are needed for each gel/membrane sandwich. Completely saturate the filter paper by soaking in transfer buffer.
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伯乐小型转膜仪（bio-radTrans-Blot）
biorad小型转印槽,biorad转印槽,伯乐转膜仪,
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【介绍】Trans-Blot 电泳转印槽组件缓冲液槽及带有电缆的盖凝胶支架转印夹纤维衬垫电极丝电极板特级冷却芯Trans-Blot 转印槽是功能灵活的转印设备，可理想地用于多种转印应用。Trans-Blot 转印槽特点包括：能进行多胶转印，可容纳3 块PROTEAN III xi 凝胶、6块Criterion 凝胶、12块Mini-PROTEAN 3 或Ready Gel 预制胶多组参数灵活可设，可调节的电压设置（从30 V 的 过夜转印到200 V 的1 小时快速实验）电极间距设置为8 cm 用于标准印迹杂交，或设置 为4cm 用于高强度印迹杂交可选择板式电极：涂有铂金的钛作为正极，不锈钢为负极，能提供高 强度电场和比其它电极更高的电流密度。或选择较经济的铂金电极丝通过特级冷却芯和水循环仪来调节温度& 是天然酶（4&C）或高强 度转印的理想选择，随着转印时间增加（多达24 小时），不会引起缓冲液耗竭（在高强度转印中必须使用冷却芯，也推荐用于所有板式 电极的应用）带铰链的凝胶支架转印夹能避免滑动，确保凝胶与印迹膜间的紧密接 触；每个转印夹都有颜色标记以保证在转印槽中的正确定位【技术指标】最大凝胶尺寸(W x L) 16 x 20 cm缓冲液要求 2.5 L凝胶容量 3 块PROTEAN II xi 凝胶、6 块Criterion 凝胶、12 块Mini-PROTEAN 3 凝胶、12 块Ready Gel 预制胶推荐电源 PowerPac HC 电源尺寸(W x L x H) 18 x 9.5 x 24 cm更多信息及产品图片请点击http://www.cbio21.net/list.asp?ProdId=0037查询！客服QQ：1410116
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伯乐转膜仪（bio-rad Trans-Blot）
Trans-Blot 电泳转印槽组件
缓冲液槽及带有电缆的盖
凝胶支架转印夹
特级冷却芯
Trans-Blot 转印槽是功能灵活的转印设备，可理想地用于多种转印应用。Trans-Blot 转印槽特点包括：
能进行多胶转印，可容纳3 块PROTEAN III xi 凝胶、6块Criterion 凝胶、12块Mini-PROTEAN 3 或Ready Gel 预制胶
多组参数灵活可设，可调节的电压设置（从30 V 的 过夜转印到200 V 的1 小时快速实验）
电极间距设置为8 cm 用于标准印迹杂交，或设置 为4cm 用于高强度印迹杂交
可选择板式电极：涂有铂金的钛作为正极，不锈钢为负极，能提供高 强度电场和比其它电极更高的电流密度。或选择较经济的铂金电极丝
通过特级冷却芯和水循环仪来调节温度& 是天然酶（4&C）或高强 度转印的理想选择，随着转印时间增加（多达24 小时），不会引起缓冲液耗竭（在高强度转印中必须使用冷却芯，也推荐用于所有板式 电极的应用）
带铰链的凝胶支架转印夹能避免滑动，确保凝胶与印迹膜间的紧密接 触；每个转印夹都有颜色标记以保证在转印槽中的正确定位
【技术指标】
最大凝胶尺寸(W x L) 16 x 20 cm
缓冲液要求 2.5 L
凝胶容量 3 块PROTEAN II xi 凝胶、
6 块Criterion 凝胶、
12 块Mini-PROTEAN 3 凝胶、
12 块Ready Gel 预制胶
推荐电源 PowerPac HC 电源
尺寸(W x L x H) 18 x 9.5 x 24 cm
更多信息及产品图片请点击http://www.cbio21.net/list.asp?ProdId=0037查询！客服QQ：1410116
所在地区：北京市 北京
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&我们会在一个工作日内给您回复！1.&Do&not&reverse&polarity&on&this&instrument
2.&Do&not&exeed&25&V&with&this&instrument.
3.&Do&not&adjust&the&pH&of&transfer&buffers
4.&Lengthy&tranfer&times&are&not&recommended.
5.&Power&supply&requirements
6.&Do&not&operate&this&instrument&in&ambient&temperatures&exceeding&50&℃.
7.&Buffer&preparation&is&extremely&important……
首先讲转膜仪的清洗：
Do not immerse the unit in liquid. Use special care when cleaning the anode plate to avoid scratching or marring the platinum. Do not use abrasives or strong detergents. The cathode plate (stainless steel) can be cleaned with a mild abrasive to remove salt that may deposit during normal operation. The entire unit can also be periodically disassembled and cleaned with water to remove salt deposits.
推荐清洗方法：转膜结束后，立即用双蒸水将转膜仪冲洗几次，并晾干。
Electrophoretic transfer of proteins and nucleic acids is dependent on many factors. Observe the following guidelines to avoid mishaps that may result in serious damage to the instrument or injury to the operator.
1. Do not reverse polarity on this instrument. This will result in corrosion and rusting of the stainless steel cathode. If this should occur, the stainless steel should be cleaned with a mild abrasive cleaner to remove the rust.
2. Do not exeed 25 V with this instrument. This could damage the electrodes.
3. Do not adjust the pH of transfer buffers unless specifically indicated. Following instructions carefully. Adjustment of pH of transfer buffers, when not indicated, will result in increased buffer conductivity. This is manifested by a higher than expected initial current output as shown by the power supply's current meter. Monitor buffer resistance with the Model 200/2.0 power supply prior to each run to insure proper buffer conductivity.
4. Lengthy tranfer times are not recommended. Do not leave this instrument unattached. Joule heat can be generated rapidly during semi-dry blotting. Transferring longer than 2 hours can damage the unit.
5. Power supply requirements. The Trans-Blot SD cell should only be used with the microprocessor-controlled Model 200/2.0 power supply or the Model
power supply.
6. Do not operate this instrument in ambient temperatures exceeding 50 ℃.
7. Buffer preparation is extremely important. Do not adjust transfer buffer pH by addition of acid or base unless specifically indicated in the instructions. Improperly prepared buffer will cause excess heat generation and safety hazards. Use only high quality, reagent grade methanol. Contaminated methanol can result in increased transfer buffer conductivity, as well as poor transfer of macromolecules.
8. Following electrophoresis, equilibrate the gels in transfer buffer. Equilibration facilitates the removal of electrophoresis buffer salts and detergents. If the salts are not removed, they will increase the conductivity of the transfer buffer and the amount of heat generated during the transfer. Also, low percentage gels (&12% acrylamide) will shrink in methanol-containing buffers. Equilibration allows the gel to adjust to its final size prior to electrophoretic transfer. The length of time required for equilibration is dependent on the gel thickness. For example, 15 minutes for a 0.75 mm -PAGE gel.
Low molecular weight macromolecules ( 10,000 daltons) may diffuse out of gels more readily. One can allow adequate gel pre-equilibration by changing the pre-equilibration buffer several times during a relatively short pre-equilibration period. This will help to limit diffusion of low molecular weight macromolecules while providing efficient salt reduction.
9. Cut the membrane to the dimensions of the gel. Wet the membrane by slowly sliding it at a 45& angle into transfer buffer and allowing it to soak for 15&30 minutes. Complete wetting of the membrane is important to insure proper binding. Abrupt wetting can lead to entrapment of air bubbles in the matrix. These air bubbles can block transfer of molecules. To avoid membrane contamination, always use forceps or wear gloves when handling membranes.
10. Cut filter paper to the dimensions of the gel. Two pieces of extra thick filter paper (or four pieces of thick or six pieces of thin filter paper) per gel are needed for each gel/membrane sandwich. Completely saturate the filter paper by soaking in transfer buffer.
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