请教MTT和trypan blue solution方法进行细胞毒性诱因的判断

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Trilex could also inhibit acetic acid induced inflammatory ooze in mice abdominal cavity. The inhibitory rate was 34.8 % .
三冬茶还可抑制醋酸所致的小鼠腹腔毛细血管通透性的提高,抑制率可达到34.8%。
Objective: To set up the mathematical model of interval estimation of the inhibitory rate of cell growth under the medical effect.
目的:建立药物作用下细胞生长抑制率区间估计的数学模型。
C1A also decreases the output of blood serum malondialdehyde (MDA) and the inhibitory rate is up to 50.00% with concentration of 15.80%.
C1A并能有效地抑制血清过氧化物丙二醛(MDA)的产生,多糖浓度达15.80%时抑制率可达50.00%。
Inhibitory rate of blood platelet aggregation.
血小板聚集抑制率。
Methods The inhibitory rate of cell growth was tested by MTT method and trypan blue stain assay in the various conditions.
方法分别采用台盼蓝拒染法和MTT比色法,在不同条件下测定细胞增殖的抑制率。
The inhibitory rate of spermatid micronucleus and spermatozoon malformation is 57.38% and 50.87% respectively.
对精细胞微核的抑制率为57.38%,对精子畸形的抑制率为50.87%。
The inhibitory rate increased with the rising of Alarelin concentrations.
随着丙氨瑞林浓度的增大,抑制率渐高。
MethodsIn vitro experiments, MTT assay was used to det ermine the cell growth inhibitory rate.
方法采用MTT比色法测定大豆异黄酮对食管癌细胞的生长抑制率;
The difference of cell growth inhibitory rate(GIR)among different cells was not significant(P>0.05).
不同细胞间细胞生长抑制率(GIR)差异无显著性(P>0.05);
The inhibitory rate of the growth in immune organs raised significantly with the prolonging of the time for feeding CTC.
随着饲用时间的持续,CTC对免疫器官生长的抑制率明显提高。
Therefore, Pre-amplification circuit should be a high input impedance, high common mode inhibitory rate, low drift Small signal enlargement electric circuit.
因此,前置放大电路应该是一个高输入阻抗,高共模抑制比、低漂移的小信号放大电路。
Mice were inoculated with B16 melanoma and L-VCR was given i. v. Tumor inhibitory rate and non-tumor body weight was calculated.
小鼠接种B16黑色素瘤,静脉给予L-VCR,计算其肿瘤抑制率以及去瘤体重;
The absorbance values of the curcumin group, TRAIL group and combination group were determined by MTT method to calculate the inhibitory rate of A549 cells.
用四氮唑蓝法测定姜黄素组、肿瘤坏死因子相关凋亡诱导配体组以及联合组的吸光值,根据吸光度值计算肺癌细胞株A549细胞生长抑制率。
After treatment, the tumor weight was measured and tumor growth inhibitory rate was calculated.
治疗结束后断颈处死裸鼠,剥瘤称重,计算抑瘤率。
Result: The ethanol extract and water extract of CKG could increase pain threshold markedly(P<0.01), the inhibitory rate was 59%, 67%, 59%, 76% accordingly.
结果:桂郁金醇提物、水提物低、高剂量对小鼠痛阈有明显的提高作用,扭体反应抑制率分别为59%,67%,59%,76%(P<0.01);
The inhibitory rate of MMC-IMAMS group on the growth of colorectal cancer xenografts was significantly higher than that of MMC-MAMS group or MMC group.
裸鼠模型体内实验丝裂霉素免疫磁性白蛋白微球组肿瘤抑制率较丝裂霉素磁性白蛋白微球组和单纯丝裂霉素组具有显著差异。
Modified fibers after lysozyme immobilized with good antimicrobial properties, inhibitory rate of 68.60%.
而改性纤维在固定化溶菌酶后具有良好的抗菌性能,抑菌率为68.60%。
The best ACE-inhibitory rate of 85 % was accomplished in the LC-15 fermented milk of a 10 %-hydrolysed degree.
控制复原脱脂乳水解度为10%,然后再利用LC-15进行发酵得到的发酵乳的ACE抑制活性达到85%;
Sp8 showed strong inhibition of angiogenesis, the inhibitory rate reached 72.7% on rabbit cornea and 79% on CAM model, respectively.
在体内具有抑制新生血管生长的作用,兔角膜模型抑制率为72 .7% ,鸡胚模型(CAM)抑制率为79% ;
Objective: To set up the mathematical model of interval estimation of the inhibitory rate of cell growth under the medical effect. Methods:Using the theory of probability and statistical.
前言:目的:建立药物作用下细胞生长抑制率区间估计的数学模型。
Results:As compared with positive drug aminoguanidine, the sera of rats treated with TSA showed significantly inhibitory effect on glycation in vitro , and the relative inhibitory rate was 102 2%.
结果:与阳性药氨基胍相比,灌服糖肾安的大鼠血清对体外糖基化反应有明显的抑制作用,相对抑制率为1 0 2 .2 %。
The inhibitory rate and growth of the tumor and the weight change of the mice were observed.
对荷瘤小鼠进行体内实验,观察该提取物对肝癌细胞生长的抑制率。
Results The cell viability, protein content, colony formation and DNA synthesis were significantly inhibited by MAPK ASO, the inhibitory rate was 52.6% , 50.5% , 54.9% and 47.5% respectively(P< 0.01).
结果:MAPK反义寡核苷酸可明显抑制细胞活力、蛋白质含量、集落形成和DNA合成,其抑制率分别为52。6%、50。5%、54。9%和47。5%,与对照组比较差异有显著性(P<0。01)。
Results Simvastain had obvious inhibitory effect on tumor growth in mice with H22 liver carcinoma, inhibitory rate for tumor growth reached over 50%.
结果辛伐他汀对小鼠的H22实体瘤生长具有明显的抑制作用,抑瘤率达50%以上。
The growth inhibitory rate of endothelial cell was determined by MTT assay;
采用MTT法,测定内皮细胞的生长抑制率;
Antioxidant activities of different extraction fractions were evaluated by scavenging capacity on DPPH free radicals and inhibitory rate of lipid peroxidation.
采用清除DPPH自由基实验和抑制小鼠肝细胞脂质过氧化活性实验检测各提取物的抗氧化活性。
Methods:The variation of both morphology and inhibitory rate of K562 cells were observed in culture medium with various concentrations of Oridonin at different time in vitro.
方法:以不同浓度的冬凌草甲素作用于体外培养的K5 6 2细胞,检测细胞生长抑制率,观察细胞凋亡时的形态学变化,对细胞凋亡前后的端粒酶活性进行检测。
Antiviral activitity of the antisense RNA was evaluated by cytopathic effect using cultured IBRS2 cell and anti- IBRS2 cell infected by TGEV respectively, and the inhibitory rate was about 50%.
通过TGEV分别感染IBRS2和IBRS2抗性细胞产生的病变,结果表明该反义RNA对病毒的复制有抑制作用,其抑制率近50%。
RESULTS Endostatin treatment alone reduced capillary-like tube numbers in vitro(inhibitory rate 33.5%) and hemoglobin content in vivo(inhibitory rate 38 . 8%) ;
结果内皮抑制素在体外抑制了管腔形成数(抑制率33.5 % ) ,在体内减少了血红蛋白含量(抑制率38.8% ) ;
Tumor weight was measured and the tumor growth inhibitory rate was calculated. Under light microscopy, the tumor morphological changes were observed.
测量瘤重、计算瘤重抑制率、制病理切片,在光镜下观察肿瘤形态与改变。
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感谢您的反馈,我们会尽快进行适当修改!产品名称: Trypan blue台盼蓝
Trypan blue台盼蓝
Trypan Blue
中文别名(Chinese
直接蓝14; 锥虫蓝;曲利苯蓝
英文别名(English
Blue 14; D Diaminine Blue
CAS号(CAS NO.)
分子式(Formula)
C34H24N6Na4O14S4
分子量(Molecular
外观(Appearance)
棕色至黑褐色、黑色、黑褐色粉末
组分(Composition)
染料含量,&40%
溶解性(Solubility)
溶于水(10mg/ml)
结构式(Structure)
0.4g100ml 0.85% NaClHank&s0.4% 1:93min
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台盼蓝拒染法、MTT法、CCK-8法在研究As2O3细胞毒性作用中的意义
目的 筛选三氧化二砷(As2O3)对HL-60细胞毒性的有效剂量及作用时间的检测方法,并对其结果进行分析.方法 采用台盼蓝拒染法、MTT法、CCK-8法测定不同浓度的As2O3体外作用HL-60细胞株的细胞存活率,采用单因素方差分析其与剂量-时间依赖反应的关系.结果 4.0、8.0μmol/L作用48、72 h及8.0 μmol/L作用24 h被染细胞小于5%,其余均未观察到蓝染细胞.MTT法检测在1.0~8.0 μmol/L As2O3剂量范围内培养24、48、72 h(P< 0.05),各组均能够抑制HL-60细胞活性.CCK-8法检测在0.5~8.0 μmol/L As2O3剂量范围内培养24、48、72 h(P< 0.05),各组均能够抑制HL-60细胞活性.HL-60细胞在不同时间、不同浓度均能出现细胞存活率明显降低,As2O3的毒副作用呈现明显的剂量-时间依赖反应.结论 在研究As2O3的细胞毒性时,用CCK-8法优于MTT法,均比台盼蓝法更为灵敏.
CHEN Chong
XU Ruirong
JING Jingyan
作者单位:
山东中医药大学第一临床学院,山东济南,250014
山东中医药大学附属医院血液病科,山东济南,250011
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国家自然科学基金资助项目
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戮穿试验,冲击试验
The Pink or Blue test, which claims 95 percent accuracy at seven weeks and gives refunds for wrong results, appears to meet the standards described in the new study.
粉红或蓝色测试,据说有在第七周有95%的准确性而且对错误结果退款,好象满足了新研究中所述的标准。
The Pink or Blue test, which claims 95 percent accuracy at seven weeks and gives refunds for wrong results, appears to meet the standards described in the new study.
“红蓝测试”宣称其结果在怀孕第七周有95%的准确率,测试错误全额退款。看上去它倒是符合新研究的标准。
It shows that the EGME and methylene blue test adsorption methods are well repeating, correct and reliable testing methods of specific surface area.
实验研究证明,EGME法和亚甲基蓝吸附法是重复性好、准确可靠的泥页岩比表面积测定方法。
An investigation is also made on determining DMDAAC qualitatively by bromophenol blue test and quantitatively by tetraphenyl boron sodium precipitation reac…
用溴酚蓝对DMDAAC进行定性分析,用四苯硼酸钠及折光率法进行定量分析的方法进行了探讨。
The well growth and the good biological activities of the retrieved PC12 cells were proved using the MTT and Trypan blue test.
回收微囊化PC12细胞再培养生长良好,MTT法和台盼兰法检测显示细胞具有生物活性。
The retrieved encapsulated PC12 cells were recultured and their activities were tested by MTT and Trypan blue test.
对移植的微囊化PC12细胞回收后再培养,以MTT和台盼兰检测细胞活性。
This will display the blue "email test" message on your screen.
这将在屏幕中显示蓝色的“emailtest”消息。
The Dutch Centre for Sustainable Water Technology (Wetsus) will also in three to four months start a pilot "blue power" test at IJsselmeer in the Netherlands, from where water flows into the sea.
与此同时,荷兰在3-4个月内也将在与海口处开展实验
Disclosure of the identity of the "shrines" Li Ya-distribution back to Tianjin, he proposed that I was into the left incite defection of blue, to test I was into.
身份暴露的“佛龛”李涯分配回到天津,他提议让余则成策反左蓝,以测试余则成。
Methods:Cell growth and differentiation were analysed by Trypan blue dye exclusion test, 7690 XU fluorescein staining and transmission electric microscopy.
方法:应用台盼蓝染色法、76 90 XU荧光染色法和电子显微镜等观察细胞生长、分化。
Should I bring a blue book for the test?
我要带蓝皮簿来应考吗?
Second, the blue print of parallel test system frame is introduced, the hardware and software design are described.
接着分别描述了并行测试系统的硬件及软件设计;
Test the blue print has designed in the security project of GanSu museum, and analyze the conclusion.
在甘肃省博物馆安防工程中对所设计的方案进行了测试,并对结论进行了分析。
HE stain, toluidine blue Dyeing and immunohistochemistry test were performed to observe the histomorphology.
行HE染色、甲苯胺蓝染色及免疫组化进行组织形态学观察。
A typical blood test like Pink or Blue, for example, costs $25 for the kit.
一个典型的血液检测,如“红蓝测试”,光试剂盒就得花上25美元。
A typical blood test like Pink or Blue, for example, costs $25 for the kit.
例如一项象红或蓝的典型测试,套件要花$25 。
There's also a light blue T-shirt with the test-color bars from TV.
还有一件浅蓝色印着电视信号测试卡的那件。
Notice that the elements turns blue, the background for the Value field changes color, and that a little blue arrow icon appears in the Test Contents tree on the response node ( Figure 16).
注意,元素变成了蓝色,Value域的背景颜色发生了改变,一些蓝色的箭头图标出现在了响应节点的TestContents树中(图16)。
Typically ERP implementations used Waterfall or vendor/implementer specific methodologies, which had phases such as Requirement, Blue Print, Build, Test/Train an Deploy.
典型的ERP系统实施采用瀑布方法或供应商、实施者的专有方法论,这些方法都有如需求、蓝图、构建、测试、培训和部署几个阶段。
The test paper turned blue.
试纸变成蓝色。
The blood in the procoagulant inert separation tube(yellow cap) was more likely to hemolysis than that in the sodium citrate coagulation test tube(blue cap).
惰性分离促凝管(黄色封盖)比枸橼酸钠凝血试验管(蓝色封盖)更易溶血。
The gasification test for producing blue gas was conducted with briquettes made by a mixture of fine coal and shift agents.
用粉煤和变换剂制成的型煤进行了水煤气气化反应试验。
And there, glowing with a faint blue light in the glass test tubes on the tables, was the mysterious something which they had worked so hard to find: Radium.
在那儿,在桌子上的玻璃试管中,正闪烁着微弱的蓝光,这就是他们付出极其艰辛的劳动所要寻找的那种神秘的东西:镭。
Lines that are highlighted light blue were exercised in the test run, and lines that are highlighted reddish brown were not.
并且会以浅蓝色显示已在执行回合中执行的程式码行,并以红褐色显示未执行的程式码行。
The Evans blue EB was used to test the permeability of blood brain barrier BBB.
用伊文思蓝测定血脑屏障的通透性。
Tubal patent test with Methylene Blue by compression can help to diagnose salpingitis, endometriosis in tubes and adenomyoma.
美蓝加压通液对诊断输卵管炎或输卵管子宫内膜异位症及子宫肌腺病有帮助。
In this test kids watched groups of blue and yellow dots flash on a computer screen, and had to guess which color was more numerous.
测试过程中,研究人员让孩子们看电脑屏幕上蓝色、黄色圆点动 画,然后让他们猜那种颜色的比较多。
To do this, she created fear memories in 65 test subjects by flashingyellow and blue squares at them, pairing some of the yellow squareswith a mild shock to the wrist.
实验中,为了制造恐惧记忆,席勒给65个实验对象观看闪烁的黄色和蓝色方块,其中黄色的方块伴有手腕的轻微电击。
To do this, she created fear memories in 65 test subjects by flashingyellow and blue squares at them, pairing some of the yellow squareswith a mild shock to the wrist.
实验中,为了制造恐惧记忆,席勒给65个实验对象观看闪烁的黄色和蓝色方块,其中黄色的方块伴有手腕的轻微电击。
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