Gel casting of hydroxyapatite with naphthalene as pore former - ScienceDirect
Export JavaScript is disabled on your browser. Please enable JavaScript to use all the features on this page., April 2015, Pages Author links open overlay panelShow moreAbstractThe present study discusses the processing and characterization of HA scaffolds which have been processed through a combination of gel casting and fugitive addition. The additional use of Napthalene (NA) as fugitive in the gel casting process provides an additional parameter for tailoring the porosity over and above that obtained through gel casting route. The motive behind the use of combination process was to prepare a porous hydroxyapatite (HA) scaffold with wide porosity range along with sufficient mechanical strength. In house prepared HA powder was used as the staring powder and Methyl acrylamide - N,N′-Methylene-bis-acrylamide (MAM-MBM) system was used for gel casting. Varying amount of Naphthalene (10–40 vol%) and HA solid loading (27–54 vol%) were used for scaffold preparation. The maximum solid loading that could be casted was 48 vol%. The sintered porous Hydroxyapatite-Naphthalene (HA-NA) scaffolds had been characterized for phase stability, microstructure, pore size and pore morphology. The highest compressive strength was 7.51 MPa recorded for 22% porosity samples, and it was 0.75 MPa for 81% porosity samples. Scaffolds prepared with lower solid loading had good pore interconnectivity. In vitro bioactivity study of the scaffolds showed dissolution of scaffold up to 3 weeks in SBF solution followed by deposition of calcium phosphate as apatite layer. MTT assay results showed acceptable limit of cell growth and proliferation.KeywordsHydroxyapatiteGel castingNaphthaleneCompressive strengthIn vitro agingMTT assayCheck if you have access through your login credentials or your institution.ororRecommended articlesCiting articles (0)Next Article in Journal
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With following keywords
preparative separation
spirobisnaphthalenes
endophytic fungus
Berkleasmium sp. Dzf12
high-speed counter-current chromatogrphy
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Tijiang Shan
Shiqiong Lu
Mingan Wang
Youliang Peng
Ligang Zhou
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Molecules 2013, 18(10), ; doi:
Preparative Separation of Spirobisnaphthalenes from Endophytic Fungus Berkleasmium sp. Dzf12 by
High-Speed Counter-Current Chromatography
MOA Key Laboratory of Plant Pathology, Department of Plant Pathology,
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China
Department of Applied Chemistry, College of Science, China Agricultural University,
Beijing 100193, China
Author to whom correspondence should be addressed.
Received: 8 July 2013 / Revised: 29 September 2013 / Accepted: 12 October 2013 / Published: 16 October 2013
[280 KB, uploaded 18 June 2014]
Abstract High-speed counter-current chromatography (HSCCC) was applied for the first time for the preparative separation of spirobisnaphthalenes from a crude extract of the endophytic fungus Berkleasmium sp. Dzf12, associated with the medicinal plant Dioscorea zingiberensis. Six spirobisnaphthalenes were successfully separated by HSCCC with a
two-phase solvent system composed of n-hexane-chloroform-methanol-water (1.5:3.0:2.5:2.0, v/v). About 18.0 mg of diepoxin k (1), 245.7 mg of palmarumycin C13 (2), 42.4 mg of palmarumycin C16 (3), 42.2 mg of palmarumycin C15 (4), 32.6 mg of diepoxin δ (5), and 22.3 mg of diepoxin γ (6) with purities of 56.82, 71.39, 76.57, 75.86, 91.01 and 82.48%, respectively, as determined by high-performance liquid chromatography (HPLC), were obtained from 500 mg of the crude extract in a one-step elution within 7 h of separation procedure by HSCCC. The purified spirobisnaphthalenes were further structurally characterized by means of physicochemical and spectrometric analysis.
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MDPI and ACS Style
Preparative Separation of Spirobisnaphthalenes from Endophytic Fungus Berkleasmium sp. Dzf12 by
High-Speed Counter-Current Chromatography. Molecules 2013, 18, .
Preparative Separation of Spirobisnaphthalenes from Endophytic Fungus Berkleasmium sp. Dzf12 by
High-Speed Counter-Current Chromatography. Molecules. ):.
Chicago/Turabian Style
Zhou, Ligang.
2013. "Preparative Separation of Spirobisnaphthalenes from Endophytic Fungus Berkleasmium sp. Dzf12 by
High-Speed Counter-Current Chromatography." Molecules 18, no. 10: .
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