鼠源r-spondin小鼠脾脏淋巴细胞分离用什么筛选

来源：蜘蛛抓取(WebSpider) 时间：2017-09-15 03:44 标签： 小鼠成纤维细胞
小鼠来源的巨噬细胞系RAW264.7细胞是什么？？用来做哪方面的研究比较适宜？_百度拇指医生
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?小鼠来源的巨噬细胞系RAW264.7细胞是什么？？用来做哪方面的研究比较适宜？
拇指医生提醒您：该问题下为网友贡献，仅供参考。
RAW 264.7小鼠单核巨噬细胞白血病细胞. 此细胞株源自雄性Abelson鼠科白血病病毒诱导的肿瘤。 sIg-, Ia-抗原、Thy-1.2表面抗原阴性。此细胞株不分泌可检测到的病毒颗粒，XC斑点形成试验阴性。 可以胞饮中性红并吞噬乳胶颗粒与酵母聚糖。 可以抗体依赖性地分解绵羊红血球与肿瘤靶细胞。 LPS或PPD处理2天可诱导分解红血球但对肿瘤靶细胞无作用。用含4mM L-谷氨酰胺，4.5g/L 葡萄糖， 1.5g/L 碳酸氢钠的DMEM培养基，90%；胎牛血清 10%培养。
………………用来做你想做的试验比较合适！太专业了
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表达单克隆抗体的重组CHO细胞筛选
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表达单克隆抗体的重组CHO细胞筛选
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All蛋白抗体ELISA Pair SetcDNAELISA Kit细胞裂解液qPCRother-->&&&&&Text Size:&&&&作者评价应用种属图像&&|&& 0">人&RSPO1&细胞裂解液产品信息表达宿主:CHO Stable Cells产品介绍:CHO lysate that Human R-Spondin 1 / RSPO1 transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).序列信息:A DNA sequence encoding the human RSPO1 (NP_) ( Met 1-Ala 263 )was expressed and purified.预测N端:Ser 21分子量:The recombinant human RSPO1 consists of 243 amino acids and has a predicted molecular mass of 26.8 kDa. As a result of glycosylation, the apparent molecular mass of it is approximately 40 and 31 kDa in SDS-PAGE under reducing conditions.分子种属:Human 0">人&RSPO1&细胞裂解液使用指导制备方法:Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.裂解缓冲液:Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.质控:12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.稳定性:Samples are stable for up to twelve months from date of receipt.使用建议:1.
Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.储存缓冲液:1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading
buffer).储存方法:Store at 4℃. After re-dissolution, aliquot
and store at -80℃.应用小提示:Western blot (WB): Use at an assay dependent dilution.Other Applications: Not tested.Optimal dilutions/concentrations should be determined by the end user. 0"> 0">R-Spondin 1/RSPO1&研究背景 RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6. 0">人&R-Spondin 1/RSPO1&参考资料 Kamata T, et al. (2004) R-spondin, a novel gene with thrombospondin type 1 domain, was expressed in the dorsal neural tube and affected in Wnts mutants. Biochim Biophys Acta. -62.Ota T, et al. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36(1):40-5.Strausberg RL, et al. (2003) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):. 货号:&11083-HNASL-300目录价:&单价:&&&&&&(You Save: )规格:数量:库存2 weeksDatasheet & DocumentationContact Us
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