ROS和MOA在化学中化学中eq是什么意思思?

上传用户:ayikhgxcga资料价格:5财富值&&『』文档下载 :『』&&『』所属分类:机构:辽宁师范大学化学化工学院,大连医科大学生理教研室基金:国家自然科学基金项目()分类号:Q-3,O657.3文献出处:关 键 词 :&&&&权力声明:若本站收录的文献无意侵犯了您的著作版权,请点击。摘要:活性氧ROS是生理过程必要的代谢产物,适量的ROS起到信号转导作用,一旦过量会对细胞、蛋白质和DNA造成损伤。在顺序注射一阀上实验室一化学发光系统内,优化并评价了鲁米诺一Co2+一ROS和鲁米诺一HRP一ROS 2种化学发光体系对ROS的检测条件和分析性能,并将其应用于肝细胞和植物叶片ROS的测定。结果表明,顺序注射一阀上实验室一ROS一化学发光检测法具有自动化程度高、分析速度快、重现性好和试剂试样用量少的显著特点,其中鲁米诺一Co2+一ROS体系灵敏度高,相对发光强度与ROS浓度在0。5~1 000nmol·L一1范围内的回归方程为Y=49 X+1 700(R2=0。992 4),检出限为0。27nmol·L一1,对细胞和植物组织中ROS的回收率在95%~104%之间,结果令人满意。Abstract:Reactive oxygen species ROS is a necessary metabolic product of the physiological process, the amount of ROS play a role in signal transduction, once excessive will cause damage to cells, proteins and DNA. A sequential injection valve in the chemical laboratory and a light emitting system, optimization and evaluation of the luminescence of luminol a CO2 + a ROS and luminol HRP a ROS two chemical system for ROS detection conditions and performance analysis and can be used in the determination of liver cells and plant leaves ROS. The results show that the sequential injection lab on valve a ROS a chemical luminescence detection method has high degree of automation, analysis speed, reproduce well and reagents consumption remarkable characteristics, the CO2 + luminol ROS sensitivity of system is high relative luminous intensity and the concentration of ROS in 0. 000nmol L 5~1 a 1 range of regression equations for X+1 Y=49 700 (R2=0. 9924), the detection limit is 0. L 27nmol 1, the recovery rate of ROS in the cell and plant tissues in the 95%~104%, the results are satisfactory.正文快照:活性氧(Reactive oxygen species,ROS)是生物体内有氧代谢过程中产生的一类化学性质活泼、氧化能力强的含氧化合物总称,包括超氧阴离子自由基O-2·、羟自由基·OH、过氧化氢H2O2等.nmol·L-1水平的ROS可促进细胞的增殖,起到细胞信号转导作用;μmol·L-1的ROS可导致细胞凋亡;mm分享到:相关文献|

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