In Utero Electroporation是什么意思,人造子宫技术内电穿孔技术翻译

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利用子宫内胚胎电转技术研究Frizzled 10在大脑皮质发育中的功能
子宫内胚胎电转技术目前被广泛应用于神经发育的相关研究.本研究利用子宫内胚胎电转的方法对Frizzled 10基因在小鼠大脑皮层发育过程中的作用作了初步的探讨.首先利用PCR扩增获得Frizzled 10的全长cDNA,将cDNA连接到电击载体pCAGIG,构建异位表达载体pCAGIG-Frizzled 10.建立子宫内电击技术:在胚鼠E 14.5 d时,将异位表达载体pCAGIG-Frizzled 10在电压35 V、脉冲时间50 ms、脉冲数5的条件下将质粒电击到背侧皮质的脑室区,4 d后收取胚鼠脑组织,进行冠状切片后在荧光显微镜下检测外源蛋白GFP在鼠胚背侧皮质的表达情况.结果显示,与对照相比,Frizzled 10的异位表达参与了皮质发育的调控.该研究为我们进一步研究Frizzled 10的功能提供了新的线索.
GU Xiao-chun
作者单位:
东南大学,生命科学研究院,发育与疾病相关基因教育部重点实验室,江苏,南京,210009
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基金项目:
国家自然科学基金,东南大学新进博士基金
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