Synegitestes express lines是什么细菌

来源：蜘蛛抓取(WebSpider) 时间：2017-03-29 00:23 标签： richard estes
Effect of probiotic bacteria on the intestinal microbiota in irritable bowel syndrome_百度文库
Effect of probiotic bacteria on the intestinal microbiota in irritable bowel syndrome
doi:10.1111/jgh.12306
GASTROENTEROLOGY
Effectofprobioticbacteriaontheintestinalmicrobiotainirritablebowelsyndrome
SiewChienNg,*EmmaFCLam,*TommyTYLam,?YawenChan,*WendyLaw,*PeteCHTse,*MichaelAKamm,+,§JosephJYSung,*FrancisKLChan*andJustinCYWu*
*InstituteofDigestiveDiseaseandDepartmentofMedicineandTherapeutics,LiKaShingInstituteofHealthSciences,ChineseUniversityofHongKong,HongK+StVincent’sHospitalandUniversityofMelbourne,Melbourne,Victoria,Aand?DepartmentofZoology,UniversityofOxford,Oxford,and§ImperialCollege,London,UK
IBS,intestinalmicrobiota,probiotics,pyrosequencing.
Acceptedforpublication12June2013.Correspondence
SiewCNg,DepartmentofMedicineandTherapeutics,PrinceofWalesHospital,Shatin,NewTerritories,HongKong.Email:siewchienng@cuhk.edu.hk
Conflictofinterest:Thereisnoconflictofinteresttobedeclared.
Authors’contribution:SCNg(spatientrecruitment,anddraftingofmanuscript),FCLam(SamplecollectionandDNAextraction),TTLam(dataanalysis,revisionofmanuscript),WTLawandCHTse(DNAextraction);MAKamm,KLChanandJYSung(revisionofmanuscript);CYWu(revisionofmanuscript).
BackgroundandAim:Inirritablebowelsyndrome(IBS),thegutmicrobiotamaybealtered.Probioticbacteriaappeartobetherapeuticallyeffective.Wecharacterizedthemucosa-associatedmicrobiota,anddeterminedtheclinicalandmicrobiologicaleffectsoforallyadministeredprobioticbacteria,inpatientswithIBS.
Methods:MucosalmicrobiotafromrectalbiopsiesofIBSpatientsandcontrolswereassessedontheV1andV2variableregionsofthe16SribosomalRNAgeneamplifiedusing454pyrosequencing.ClinicalsymptomsandchangesinmucosalmicrobiotawereassessedinIBSpatientsbeforeandafter4weeksoftreatmentwithprobioticmixVSL#3.Results:TenIBSsubjects(meanage46years)wereincluded.Atweek4ofprobiotictherapy,sixpatientsshowedsymptomimprovementonglobalsymptomassess-mentcomparedwithbaseline(P=0.031).Beforetherapy,intestinalmicrobiotaofIBSsubjectsdifferedsignificantlyfromthatofhealthycontrols,withlessdiversityandeven-nessthancontrols(n=9;P&0.05),increasedabundanceofBacteroidetes(P=0.014)andSynegitestes(P=0.017),andreducedabundanceofActinobacteria(P=0.004).TheclassesFlavobacteria(P=0.028)andEpsilonproteobacteria(P=0.017)werelessenrichedinIBS.Abundancedifferenceswerelargelyconsistentfromthephylumtogenuslevel.ProbiotictreatmentinIBSpatientswasassociatedwithasignificantreductionofthegenusBacteroides(P&0.05)tolevelssimilartothatofcontrols.Conclusion:Inthispilotstudy,globalanddeepmolecularanalysisdemonstratesanalteredmucosalmicrobiotacompositioninIBS.Probioticleadstodetectablechangesinthemicrobiota.Theseeffectsofprobioticbacteriamaycontributetotheirtherapeuticbenefit.
Background
Irritablebowelsyndrome(IBS)affectsupto20%oftheadultpopulations.1Thedevelopmentofeffectivetherapieshasbeenhamperedbyapoorunderstandingofdiseaseetiology.Thepatho-genesishasbeenthoughttorelatetochangesinbrain-gutaxisactivity2butemergingevidencesuggeststhat,atleastinasub-groupofpatients,peripheralmechanismsinthegut,includinglow-gradeinflammation,abnormalimmuneactivation,andalteredvisceralsensitivity,mayplayanimportantroleinsymptomdevelopment.3
Withtheadventofmoleculartoolsforin-depthanalysisofbacteriacommunities,studiessuggestthattheintestinalmicro-biotamayalsoplayasignificantpathophysiologicalrole.4InsupportofthisistheobservationthatpostinfectiousIBSdevelopedinasubgroupofpatientsfollowingprovengastroenteritis,5–7andthatfecalmicrobiotaarealtered.8Furthermore,IBSsymptomsareimprovedbytherapeuticinterventionsthattargetthemicrobiota
includingantibiotics,probiotics,andprebiotics.9–11Nonetheless,moststudiesinhumanaredescriptive,anditremainsunclearwhetheralteredmicrobiotaisacauseoreffectofIBS.
Muchofthedatarelatedtothenatureofchangesinthemicro-biotainIBShavebeeninconsistentbecauseoflimiteddepthorpoweroftheanalysisandpatientheterogeneity.MoststudieshavestudiedfecalmicrobiotainIBSwhichislikelytodifferfrommucosalmicrobiota.12Themechanismsofactionofprobioticsarealsonotclear.Thisstudyaimedtoinvestigatemucosa-associatedmicrobiotacompositioninIBSusingdeeppyrosequencingandlarge-scaledataanalysis,andtocharacterize,toourknowledgeforthefirsttime,thetherapeuticeffectsofprobioticsonmucosamicrobiotainIBS.
Materialandmethods
Subjects.Inthissingle-center,open-label,proof-of-conceptstudy,consecutivepatientsaged18orabovewithadiagnosis
JournalofGastroenterologyandHepatology28(–1631
(C)2013JournalofGastroenterologyandHepatologyFoundationandWileyPublishingAsiaPtyLtd
贡献者：yxliu5
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