British Library EThOS: The role of stroma microenvironments in prostate cancer cell migration and metastasis
application.name
Use this URL to cite or link to this record in EThOS:
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.561072
The role of stroma microenvironments in prostate cancer cell migration and metastasis
Lakins, Matthew
Awarding Body:
University of York
Current Institution:
University of York
Date of Award:
Availability of Full Text:
Access through EThOS:
Access through Institution:
Terminal prostate cancer is the result of metastatic spread of the tumour from the prostate and is the 2nd leading cause of cancer deaths in men. Although in vitro assays have been developed to screen for inhibitors of prostate cancer metastasis and cell migration, they routinely utilise 2- Demensional (2-D) culture of prostate cancer cell lines. Current assays do not simulate complexity of the in vivo human tumour microenvironment which contains a mixture of normal and transformed epithelial cells and stromal cells. Therefore there is a requirement to develop novel 3-D models to recapitulate the in vivo tumour microenvironment to understand tumour stroma function. Utilising a 3-D co-culture spheroid model incorporating primary human tumour stroma with prostate cancer cells we have shown that the stroma has a key role in prostate cancer epithelial cell migration and motility. By utilising 4-D two-photon imaging and gene expression analysis we have analysed the molecular mechanisms of stromal cell mediated prostate cancer cell migration, identifying genes that regulate the migration process. Analysis of human tumour stroma indicates a key role in the immune system in driving tumour stroma to express a lymphoid stromal phenotype that provides the microenvironment for active tumour cell migration. This offers an interesting dichotomy that the formation of lymphoid like stroma in aggressive tumours may paradoxically deliver help to drive the immune response to the tumour whilst simultaneously providing the microenvironment for tumour cell migration and metastasis. The molecular mechanism of prostate cancer cell migration and metastasis and stroma-epithelial cell interactions involves a complex balance between the adhesion molecule VCAM-1 and two counter ligands VLA-4 and SPARC, also known as osteonectin or BM-40. The utilisation of shRNA knockdown, Fc chimeric proteins and blocking antibodies, indicates a key role for stromal-epithelia adhesion and detachment dependent cell migration mediated by VCAM-1, VLA-4 and SPARC.
Supervisor:
Coles, Mark
Not available
Qualification Name:
Thesis (Ph.D.)
Qualification Level:
uk.bl.ethos.561072&
Not availableRegulation of Cell Migration in Metastasis of Breast Cancer--《Chinese Journal of Cell Biology》2008年01期
Regulation of Cell Migration in Metastasis of Breast Cancer
Xiao-Hui Chen, Yu-Mei Feng (Department of Biochemistry and Molecular Biology, Breast Cancer Prevention and Treatment Key Laboratory of Ministry of Education, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China)
Cell migration is one of the crucial steps in breast cancer invasion and metastasis. In the process of cell migration, acquisition of the capability of directed migration forced by the reorganization of actin cytoskel- eton in regulation of Rho family small GTPases is the primary mechanism. In addition, pleasant local microenviron- ment provided by the interaction of breast cancer cells and extracellular matrix makes cell migration convenient. Finally, cancer cells are attracted and arrested at the target organs, and proliferate into metastases. Thus, researches of the molecular mechanisms in cell migration may provide novel strategy for anti-metastasis therapy of breast cancer.
【Key Words】：
【Fund】：
【CateGory Index】：
supports all the CNKI
only supports the PDF format.
【References】
Chinese Journal Full-text Database
HUANG Pei-de1,HUANG Fei -cheng2,CHEN Yang1,XIAO Gang1,ZHOU Zhi-you1,WANG Ju1,ZHOU Tian-hong3 (1Bioengineering Institute,2Chemistry Department,3Biology Department,Jinan University,Guangzhou 510632,China.);[J];Chinese Journal of P2010-05
【Co-references】
Chinese Journal Full-text Database
NING Jia,WANG Deping,HUANG Wenhai,ZHOU Nai,YAO Aihua(School of Materials Science and Engineering, Tongji University, Shanghai 200092);[J];Materials R2006-12
LIN Dan-ying,WANG Xiu-feng,HAN Dong,MA Wan-yun,CHEN Die-yan ,Department of Physics, Tsinghua University,Beijing 100084,China);[J];Journal of Chinese Electron Microscopy S2004-02
Bao Xingfeng, Fang Jinian (Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 2000031);[J];CHINESE JOURNAL OF ANALYTIEAL CHEMISTRY;2000-10
MA Dong-yang , XUE Zhen-xun , MAO Tian-qiu . Department of Stomatology , Lanzhou General Hospital of PLA , Lanzhou 730052 , C[J];Foreign Medical Biomedical Engineering F2004-01
Liu Yanhui et al.;[J];Science & Technology R2003-03
RUAN Xiang-yuan1,TAN Yan2,CHEN Xiao-ming2(1.Department of Chemical Biotechnology,Dongguan University of Technology,Dongguan .Faculty of Chemistry,Xiangtan University,Xiangtan 411105,China);[J];Journal of Instrumental A2007-01
HU Ming-qian,CAI Ji-ye (Chemistry Department,Jinan University,Guangzhou 510632,China);[J];Journal of Instrumental A2008-12
WANG Jiong-kun1,HU Ming-qian2,XING Fei-yue11Institute of Tissue Transplantation and Immunology, 2Chemistry Department,Jinan University, Guangzhou 510632, C[J];Chinese Journal of Cellular and Molecular I2008-10
HUA Yun-peng,LI Shao-qiang,LIU Jie,PENG Bao-gang,LUO Can-qiao,LIANG Li-[J];Chinese Journal of P2009-10
Yuan Xia Liu G[J];Chinese Journal of Clinical O2002-10
【Secondary References】
Chinese Journal Full-text Database
YANG Shu-Yan,YANG Bao-Li,LI Shao-Hua.The Fourth Affiliated Hospital of Harbin Medical University,Harbin 150086,Heilongjiang,C[J];Maternal and Child Health Care of C2011-19
Similar Journals
(C)2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved